Description
Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain(Habc), a SNARE domain(known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein(1-226aa) contains N-terminal domain(Habc) and t_SNARE domain(H3 domain) and this protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques